Analysis of the mechanisms of embryogenesis through carrot somatic embryogenesis
نویسندگان
چکیده
منابع مشابه
The effects of calcium and magnesium on carrot (Dacus carrota cv. Nants) petiole somatic embryogenesis
The present study was conducted in two different experiments based on complete randomized design to evaluate the effects of calcium (0, 1, 2, 4 mM) and magnesium (0, 1.02., 2.04, 4.08 mM) on carrot petiole somatic embryogenesis in B5 medium. A difference on somatic embryogenesis was observed among different levels of calcium and magnesium. The number of globular embryos in medium with low magne...
متن کاملDifferential Accumulation of Biotin Enzymes during Carrot Somatic Embryogenesis.
The activities of four biotin enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-met...
متن کاملSynchronization of somatic embryogenesis in a carrot cell suspension culture.
Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...
متن کاملZygotic embryogenesis versus somatic embryogenesis
This review will summarize molecular and genetic analyses aimed at identifying the mechanisms underlying the sequence of events during plant zygotic embryogenesis. These events are being studied in parallel with the histological and morphological analyses of somatic embryogenesis. The strength and limitations of somatic embryogenesis as a model system will be discussed briefly. The formation of...
متن کاملSomatic Embryogenesis
Somatic embryogenesis is an asexual form of plant propagation in nature that mimics many of the events of sexual reproduction. Also, this process may be reproduced artificially by the manipulation of tissues and cells in vitro. Some of the most important factors for a successful plant regeneration are the culture medium and environmental incubation conditions. In vitro somatic embryogenesis is ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 1998
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.15.supplement_1